Proteomics

The main objective of the IdISBa Proteomics platform is to foster scientific research and technological development in the field of proteomics, enabling studies into quantitative proteomics and protein-protein interactions, as well as characterising differential expressions and post-translational modifications.

Equipment:

LAS 4000 chemiluminescence imager (General Electric).
FLA 9500 fluorescence scanner (General Electric).
Odyssey CLX infrared scanner (LI-COR).
MAGPIX Luminex unit (Millipore).
2 EPS-601 electrophoresis power supplies (General Electric).
Mini VE vertical electrophoresis system (General Electric).
2D Ettam Dalt Six electrophoresis system (General Electric).
ETTAM IPGphor 3 isoelectric focusing system (General Electric).
TransBlot Turbo transfer system (BioRad).
Duomax 1030 rocker shaker (Heidolph).
Multimode plate reader Biotek Synergy H1.
Nanoparticle size and concentration distribution scanner Nanosight NS300.

Portfolio of services:

The main applications offered by the IdISBa in the field of proteomics are:

One-dimensional and two-dimensional electrophoretic protein separation.
Differential expression analysis using chemiluminescence or infrared (Western blotting) and by DIGE.
Quantification through multiplex techniques (Luminex).
Training users in handling the equipment available in the Platform.
Technical consulting in experimental design, preparing samples, image analysis, etc..

Location:

The Proteomics platform is located in Module I on level -1 at Son Espases University Hospital. It has a floor area of 19.4 square metres.

Personal Proteómica