Services and Rates | Genomics and Bioinformatics
Services and Rates
Service Portfolio
The main applications currently offered by IdISBa in the field of genomics include:
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Digital PCR QX200 (BioRad):
- Absolute quantification of DNA or RNA
- Genotyping and mutation detection
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Bioanalyzer 2100 and TapeStation 4200 (Agilent):
- Quality control and quantification of DNA and RNA samples
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MiSeq NGS Sequencer (Illumina):
- 15 Gb and 25 M reads per run
- DNAseq and RNAseq panel sequencing
- Library preparation and QC for NGS
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Affymetrix Array Technology Unit:
- RNA expression profiling (coding and noncoding)
- microRNA expression profiling
- Isoform expression/splicing studies
- Highresolution cytogenetic studies for loss/gain and loss of heterozygosity
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Realtime qPCR systems: CFX96 Touch, CFX Connect, LightCycler 480
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Pulsedfield gel electrophoresis: Chef Mapper XA
Bioinformatics Analysis and Support
- Differential expression analysis of genes, miRNAs and lncRNAs
- miRNA-mRNA expression correlation analyses
- RNAseq analysis (fastQC, multiQC, STAR, featureCounts, DESeq2)
- Singlecell RNAseq: full Seurat pipeline (preprocessing, clustering, integration, visualization, CellPhoneDB)
- Gene coexpression network analysis (WGCNA)
- Biological significance analysis: ontology annotation, functional enrichment, signalling pathways
- Results interpretation
- Flexible cloud computing environments (CPU, RAM, GPU, SSD)
- Cloud storage for fast access and longterm archiving
- Software development and implementation: custom applications for analysis and visualization
- Support with version control and Git/GitHub repository management
- Technical and bioinformatic advisory